Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin

Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin | bioRxiv Skip to main content Home About Submit ALERTS / RSS Search for this keyword Advanced Search New Results Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin Samirul Bashir , Xi-Ming Sun , Yixuan Zhao , Nuria G. Martínez-Illescas , Isabella M. Gallego Lopez , Lauren Guerrero Negrón , Makoto Ohiro , Daniel Keifenheim , Tatiana Karadimitriou , Mary Pickering , Samuel Marguerat , View ORCID Profile Nicholas Rhind doi: https://doi.org/10.1101/2023.01.16.524304 Samirul Bashir 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA Find this author on Google Scholar Find this author on PubMed Search for this author on this site Xi-Ming Sun 2 MRC Laboratory of Medical Sciences (LMS) , Du Cane Road, London W12 0NN, UK 3 Institute of Clinical Sciences (ICS), Faculty of Medicine, Imperial College London , Du Cane Road, London W12 0NN, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site Yixuan Zhao 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA 4 The Rockefeller University , 1230 York Avenue, New York, NY 10065, USA Find this author on Google Scholar Find this author on PubMed Search for this author on this site Nuria G. Martínez-Illescas 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA 5 Present address: Department of Biochemistry and Molecular Biology, School of Biology, Complutense University , Madrid, Spain Find this author on Google Scholar Find this author on PubMed Search for this author on this site Isabella M. Gallego Lopez 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA 6 VIB-KU Leuven Center for Brain & Disease Research , 3000 Leuven, Belgium Find this author on Google Scholar Find this author on PubMed Search for this author on this site Lauren Guerrero Negrón 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA 7 Universidad Central del Caribe School of Medicine , Bayamón, PR 00960 Find this author on Google Scholar Find this author on PubMed Search for this author on this site Makoto Ohiro 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA Find this author on Google Scholar Find this author on PubMed Search for this author on this site Daniel Keifenheim 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA 8 Department of Genetics, Cell Biology and Development, University of Minnesota , Minneapolis, MN 55455, USA Find this author on Google Scholar Find this author on PubMed Search for this author on this site Tatiana Karadimitriou 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA 9 USC Keck School of Medicine , 1975 Zonal Avenue, Los Angeles, CA 90033, USA Find this author on Google Scholar Find this author on PubMed Search for this author on this site Mary Pickering 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA 10 RNA Therapeutics Institute, University of Massachusetts Medical School , 368 Plantation Street, Worcester, MA 01605, USA Find this author on Google Scholar Find this author on PubMed Search for this author on this site Samuel Marguerat 2 MRC Laboratory of Medical Sciences (LMS) , Du Cane Road, London W12 0NN, UK 3 Institute of Clinical Sciences (ICS), Faculty of Medicine, Imperial College London , Du Cane Road, London W12 0NN, UK 11 Genomics Translational Technology Platform, UCL Cancer Institute, University College London , Gower Street, London, WC1E 6BT, UK Find this author on Google Scholar Find this author on PubMed Search for this author on this site Nicholas Rhind 1 Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, MA 01605, USA Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Nicholas Rhind For correspondence: nick.rhind{at}umassmed.edu Abstract Full Text Info/History Metrics Preview PDF Abstract The major fission yeast cyclin, Cdc13, has been shown to increase in concentration in correlation with cell size, and has been proposed to thereby regulate cell size at division. However, the mechanism of its cell-size regulation has been unknown. Here, we show that Cdc13 is regulated post-transcriptionally. Its transcript is not expressed in a size-dependent manner, rather a size-dependent concentration of protein is expressed from a size-independent concentration of mRNA. Moreover, we show that the expression of Cdc13 is, in fact, size dependent, as opposed to simply size-correlated due to time-dependent expression. We identify a 20-amino-acid motif, which includes the APC D-box degron, as necessary and sufficient for size-dependent expression, which allowed us to construct a size-independent allele of cdc13 . Using this allele, we show that size-dependent expression of Cdc13 is not required for size control in fission yeast cells. Competing Interest Statement The authors have declared no competing interest. Footnotes Structure-function analysis of Cdc13 expression has extended. Funder Information Declared NIGMS , GM148359 Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license . Back to top Previous Next Posted March 25, 2026. Download PDF Email Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. You are going to email the following Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin Message Subject (Your Name) has forwarded a page to you from bioRxiv Message Body (Your Name) thought you would like to see this page from the bioRxiv website. Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. Share Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin Samirul Bashir , Xi-Ming Sun , Yixuan Zhao , Nuria G. Martínez-Illescas , Isabella M. Gallego Lopez , Lauren Guerrero Negrón , Makoto Ohiro , Daniel Keifenheim , Tatiana Karadimitriou , Mary Pickering , Samuel Marguerat , Nicholas Rhind bioRxiv 2023.01.16.524304; doi: https://doi.org/10.1101/2023.01.16.524304 Share This Article: Copy Citation Tools Post-Transcriptional Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin Samirul Bashir , Xi-Ming Sun , Yixuan Zhao , Nuria G. Martínez-Illescas , Isabella M. Gallego Lopez , Lauren Guerrero Negrón , Makoto Ohiro , Daniel Keifenheim , Tatiana Karadimitriou , Mary Pickering , Samuel Marguerat , Nicholas Rhind bioRxiv 2023.01.16.524304; doi: https://doi.org/10.1101/2023.01.16.524304 Citation Manager Formats BibTeX Bookends EasyBib EndNote (tagged) EndNote 8 (xml) Medlars Mendeley Papers RefWorks Tagged Ref Manager RIS Zotero Tweet Widget Facebook Like Google Plus One Subject Area Cell Biology Subject Areas All Articles Animal Behavior and Cognition (7998) Biochemistry (18705) Bioengineering (14824) Bioinformatics (44323) Biophysics (22545) Cancer Biology (19664) Cell Biology (26832) Clinical Trials (138) Developmental Biology (13929) Ecology (20965) Epidemiology (2067) Evolutionary Biology (25405) Genetics (16143) Genomics (23459) Immunology (18664) Microbiology (42377) Molecular Biology (18015) Neuroscience (93278) Paleontology (699) Pathology (2976) Pharmacology and Toxicology (5082) Physiology (8097) Plant Biology (15963) Scientific Communication and Education (2094) Synthetic Biology (4546) Systems Biology (10214) Zoology (2382)

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