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Agro_AD1484_piliprep7_20150916

Ghosal, Debnath
CaltechDATA · Papers · License: CC BY-NC 4.0
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agrobacterium-tumefacienspipeline
AD1484:A348dB/pAD2079, virB on a multicopy-plasmidFor pili prep, following buffer was used: 20 mM Tris-HCl, pH 7.8, 100 mM NaCl and 0.5% deoxycholate. Did not add any gold. I assumed we will only do high resolution imaging. However, later we decided to do tomography and used patch tracking to proces
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