Artificial Enzyme Design with an Abiological Salicylaldehyde Site Enables Biocatalytic Activation of Glycine Esters

Here we describe the construction of an artificial enzyme bearing a biomimetic PLP-like catalytic center within an apo-myoglobin scaffold through covalent incorporation of an abiological salicylaldehyde moiety. By mimicking the substrate activation strategy employed by PLP-dependent enzymes, the eng