bioRxiv / medRxiv
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CC BY 4.0
Pre-FIB Layer-Mapping Cryo Tomography (PLCT) for Depth-Resolved in Situ Structural Analysis of Multilayered Tissues
Cryo-electron tomography (cryo-ET) enables near-native visualization of subcellular architectures, yet applying it to moderately thick, multilayered tissues such as the retina is hampered by inadequate vitrification and inaccurate depth-targeting. Here, we developed PLCT, an integrated approach comb
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