Human Immunity Against Staphylococcus Aureus Skin Infection

Background: \- Staphylococcus aureus, or staph, is commonly found on the skin and in the respiratory system. Sometimes people who get sick with staph infection do not get better with standard treatment. These staph infections can be serious and even deadly. Researchers want to find out why some people are more likely to get the infection. Objectives: \- To look at the immune response of the skin when it is exposed to bacteria. Eligibility: * People age 2 65 with hyper IgE syndrome (HIES) and those with recurrent staph infections. * Healthy volunteers. Design: * Participants will be screened with medical history, physical exam, and blood tests. * Over 1 5 days, participants may have blood tests and a skin and nasal swab. They may have additional tests if needed. If they had a recent biopsy, researchers may ask for a sample from it. * Some participants will spend the night at the clinic. Their vital signs will be taken and they will have blood drawn. Some participants will take aspirin or ibuprofen starting 2 days before their stay. * Some participants will have blisters created on the inside of their forearm. Suction will pull a layer of skin from their arm. Skin will be removed. Different solutions will be applied to the blisters. Up to 3 biopsies may be taken. * Children will not have blood tests or biopsies. * Participants will be called every day for 10 days, then at 30 days after the procedure. * Participants will have a follow-up visit 10 days after the procedure. * Participants who did not get blisters or biopsies will not have any follow-up appointments.