HAL (France)
open access
Single-molecule force measurements show that r-proteins assist 23S rRNA co-transcriptional folding
Mechanical overstretching of individual RNA-DNA hybrids is used as a novel in vitro assay to prepare a co-transcriptional RNA structure and study its interaction with proteins. Dual optical traps hold two microscopic beads linked by an RNA-DNA molecular construct that is designed such that the RNA s
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