HAL (France)
open access
Combining membrane potential and calcium imaging in brain slices using the voltage-sensitive dye ElectroFluor630 and the calcium indicator Calbryte520
SignificanceWide-field imaging from brain slices stained with a voltage-sensitive dye (VSD) and simultaneously loaded with a Ca2+ indicator allows investigating neuronal excitability and synaptic transmission at a multi-cellular scale. So far, achieving this combined imaging has been limited by expe
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