bioRxiv / medRxiv
open access
CC BY 4.0
SPARKLE: evidence-constrained correction of local RNA leakage in high-resolution spatial transcriptomics
High-resolution sequencing-based spatial transcriptomics, including Stereo-seq and Visium HD, aggregates dense capture units into cell-resolved expression matrices. During tissue processing and permeabilization, RNA released from source cells can spread to neighbouring capture locations, reducing ce
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