bioRxiv / medRxiv
open access
CC BY 4.0
A pseudohelicase-centered complex couples assembly-dependent RNA cleavage to poly(UG)ylation
Robust RNA interference in Caenorhabditis elegans requires Argonaute targeted RNAs to be cleaved by RDE 8 and poly(UG)ylated by MUT 2, generating templates for RNA dependent RNA polymerases that synthesize secondary small RNAs. RDE 8 and MUT 2 are physically coupled within the cleavage and poly(UG)y
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