PLOS
open access
CC BY 4.0
A modular toolkit for theophylline-dependent synthetic auxotrophs via riboswitch-gated essential genes
Synthetic auxotrophs are a useful means for developing genetically encoded biocontainment systems. Current methods for developing synthetic auxotrophs are complicated or expensive, limiting access and adoption of biocontainment technologies. To address this gap, we developed a simple and modular pl
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