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A membrane-free spot-plating protocol for <i>Agrobacterium</i>-mediated transformation of diverse yeasts
Agrobacterium-mediated transformation (AMT) is a critical method for genetic manipulation of non-model fungi, yet it remains a laborious and inefficient technique. When co-cultured in acetosyringone-supplemented induction medium, Agrobacterium transfers DNA directly into yeast cells using its virul
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