Highly Multiplexed TAG-Mass MALDI-MSI Dataset for Spatial Immune Profiling of Breast Tumors | Zenodo Skip to main Communities My dashboard Log in Sign up Published April 23, 2026 | Version v1 Dataset Open Highly Multiplexed TAG-Mass MALDI-MSI Dataset for Spatial Immune Profiling of Breast Tumors Authors/Creators Zirem, Yanis (Data manager) Salzet, Michel (Supervisor) Fournier, Isabelle (Supervisor) 1 Show affiliations 1.
PRISM U1192 Description Dataset description (MALDI-MSI TAG-mass panel): This dataset contains MALDI mass spectrometry imaging (MSI) data acquired across multiple acquisition cycles, where each cycle corresponds to a panel of antibody-derived mass tags (TAGs). Each immunohistochemical marker is conjugated to a unique mass reporter, allowing multiplexed detection through its specific mass-to-charge ratio (m/z). For each cycle: Cycle 1 includes markers such as CD4 (m/z 192.266), CD8 (215.200), FoxP3 (189.185), PD-L1 (203.222), and Ki67 (245.293), targeting T-cell populations, immune checkpoints, and proliferation. Cycle 2 focuses on co-stimulatory and regulatory receptors, including CD28 (189.215), CD40 (192.266), OX40 (217.279), and CTLA4 (231.246). Cycle 3 captures antigen presentation and exhaustion-related markers such as MHC I (215.200), MHC II (219.252), LAG3 (192.266), and HLA-E (245.293). Cycle 4 includes activation and inhibitory receptors such as CD137/4-1BB (231.286), NKG2A (192.266), and TIGIT (233.337). Cycle 5 contains markers related to immune regulation and suppression, including CD154/CD40L (219.315), VISTA (205.273), and IDO II (245.310). Each m/z value uniquely identifies a tagged antibody, enabling spatially resolved detection of marker expression in tissue sections. Prognostic annotations (good, neutral, or bad) and biological mechanisms associated with each marker are also provided in the table to facilitate biological interpretation. The imzML files deposited here correspond to individual acquisition cycles, and users can reference the provided marker–m/z mapping table to interpret ion images and reconstruct multiplexed protein distributions. Files Files (20.6 GB) Name Size Cycle1.ibd md5:ba7cec5012f37f72b8c759363b076b3b 3.2 GB Download Cycle1.imzML md5:a8ca1542c0437ee2b21b0efd637fe540 918.3 MB Download Cycle2.ibd md5:4c7f1e781151720c37896ba77ba9ff11 3.3 GB Download Cycle2.imzML md5:679fe98a65a5df211e74e8f0a818ed92 938.3 MB Download Cycle3.ibd md5:a6f53f4a1de3812c16baed3142995e31 3.2 GB Download Cycle3.imzML md5:bd2bab949e15d37fcab5a6ac7164d922 915.4 MB Download Cycle4.ibd md5:ec009c7439e8e4f7d3ab5fabb50199e7 3.2 GB Download Cycle4.imzML md5:777d28754e30a57e517d1d0c454c0ed7 917.7 MB Download Cycle5.ibd md5:09a97f81d3c958acc428a63a70d8fa3e 3.1 GB Download Cycle5.imzML md5:4a4b0a367b9a68076fb5e2bcc7ef9c6c 899.8 MB Download marker_table.html md5:58168f0a8c72862c947b7ccfba3819b7 25.9 kB Download Additional details Additional titles Other A Universal Modular High-Plex MALDI Mass Spectrometry Imaging Platform Funding Agence Nationale de la Recherche Click&Detect 23-CE29-0018-01 78 Views 36 Downloads Show more details All versions This version Views Total views 78 78 Downloads Total downloads 36 36 Data volume Total data volume 58.6 GB 58.6 GB More info on how stats are collected.... Versions External resources Indexed in OpenAIRE Communities Details DOI DOI Badge DOI 10.5281/zenodo.19705736 Markdown [](https://doi.org/10.5281/zenodo.19705736) reStructuredText .. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.19705736.svg :target: https://doi.org/10.5281/zenodo.19705736 HTML <a href="https://doi.org/10.5281/zenodo.19705736"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.19705736.svg" alt="DOI"></a> Image URL https://zenodo.org/badge/DOI/10.5281/zenodo.19705736.svg Target URL https://doi.org/10.5281/zenodo.19705736 Resource type Dataset Publisher Zenodo Published in A Universal Modular High-Plex MALDI Mass Spectrometry Imaging Platform, 2026. Rights License Creative Commons Attribution 4.0 International The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited. Read more Citation Export Technical metadata Created April 24, 2026 Modified April 28, 2026 Jump up About About Policies Infrastructure Principles Projects Roadmap Contact Blog Blog Support Help FAQ Developers REST API OAI-PMH Contribute GitHub Donate Funded by Powered by CERN Data Centre & InvenioRDM Status Privacy policy Cookie policy Terms of Use This site uses cookies. Find out more on how we use cookies Accept all cookies Accept only essential cookies