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Biochemical characterisation of fungal bioluminescence enzymes reveals substrate inhibition and secondary turnover of 3-hydroxyhispidin by H3H

Burnett, J. F. et al.
bioRxiv / medRxiv · Papers
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biochemistry
The fungal bioluminescence pathway enables autonomous light production from caffeic acid and has emerged as a versatile platform for bioimaging and synthetic biology. However, biochemical characterisation of its core enzymes remains limited. Here, we establish recombinant purification and quantitati
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