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A rapid and direct method for the determination of active site accessibility in proteins based on ESI-MS and active site titrations

Norah O'Farrell (25360695) et al.
Figshare · Datasets
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We have developed an electrospray ionisation mass spectrometry (ESI-MS) technique that can be applied to rapidly determine the number of intact active sites in proteins. The methodology relies on inhibiting the protein with an active-site irreversible inhibitor and then using ESI-MS to determine the
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