HAL (France)
open access
Live-cell quantitative FRET imaging made simple by autocalibration in QuanTI-FRET
Genetically-encoded biosensors based on the Fluorescence Resonance Energy Transfer (FRET) between two fluorescent proteins have the power to measure biochemical activity in living cells with the spatio-temporal resolution given by optical microscopy. The generalization of their usage is limited by t
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