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Expanding N-terminomics Coverage by Extending N-terminal Peptide Length

Takeshita, A. et al.
bioRxiv / medRxiv · Papers
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biochemistry
In bottom-up proteomics, protease specificity determines peptide length and LC/MS/MS detectability; in N-terminomics, this is critical because each protein N-terminal peptide is unique and cannot be substituted by other peptides. CHromatographic AMplification of Protein N-terminal peptides (CHAMP-N)
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