40733 | Zenodo Skip to main Communities My dashboard Log in Sign up Published April 22, 2026 | Version 1 Output management plan Open 40733 Authors/Creators KOUSHIK ROY (Project member) 1 Show affiliations 1.
ARGOS Contributors Researcher (2): KOUSHIK ROY (0000-0001-7205-9034) 1 Oscar Monroig (0000-0001-8712-0440) 1 Show affiliations 1.
orcid Description Project investigates a two-phase, balanced pond-feeding strategy that uses agri-food by-products to spare natural zooplankton/benthos, curb eutrophication, and enrich EPA+DHA in common carp. This basic research would help understand the dynamic nature of LC-PUFA accumulation bio-machinery existing in European fishponds, where low-trophic level fish such as common carp are co-feeding on natural food and different supplementary feed, that easily shift between ketogenic- and starchy- diet typologies under seasonally changing background levels of natural food. This proposal aims to evaluate the impact of a newly developed balanced pond feeding strategy to suppress eutrophication (i.e., combining plankton ecology and fish nutritional bioenergetics), on LC-PUFA bioaccumulation in Cyprinus carpio (common carp) under Central European modified-extensive pond aquaculture. Results will provide molecular evidence for regenerative pond aquaculture. Study aim and objectives Our overarching aim is to understand and characterize the biomachinery of temperate European fishponds that supports land-locked EPA and DHA production, comparing traditional versus improved pond feeding practices based on regenerative or restorative principles aimed at reversing eutrophication in standing waterbodies. In this proposal, we aim to gain molecular insights into the responses of common carp to different feeding regimes in the pond, focusing on the activation of lipid metabolic processes such as de novo lipogenesis and LC-PUFA biosynthesis. The specific objectives include: 1. Investigate the dietary regulation of de-novo lipogenesis and LC-PUFA biosynthesis gene markers, as well as their transcription factors. 2. To clarify the endogenous capacity of the common carp to biosynthesize DHA via the Sprecher pathway. Experimental plan In total, 32 cDNA samples have been synthesized from total RNA samples prepared from liver of carps fed four diets (4 diets x 8 fish), following the instructions detailed in INVITROGEN kits for RNA extraction and cDNA synthesis in a RNAZap cleansed and UV-sterilized molecular biology lab using RNA-ase free consumables. The cDNA samples will be shipped to Dr. Oscar Monroig (IATS-CSIC, Spain), requesting gene expression analytical services from his group. While the exact number of genes is yet to be determined, approximately 10-12 genes are expected to be analyzed by quantitative PCR (qPCR). These include markers of de-novo lipogenesis (e.g., Fas, Acaca, Scd1) and LC-PUFA biosynthesis (Fads2, Elovl2, Elovl5), and transcription factors (TF) (e.g., Srebp1, Ppara) regulating their expression. To normalize the gene expression results, the expression of potential reference genes (e.g., Ef1a, 18S) will be also measured, so that only stable genes will be utilized for normalization purposes. As detailed above, a second objective of this proposal consists in determining the role that the common carp Fads2 desaturases play in the DHA biosynthesis via the Sprecher pathway. A yeast-based functional assay will be used as described by Oboh et al. (2017). Briefly, transgenic yeast expressing either of the two common carp Fads2 will be incubated in the presence of 24:5n-3. After 2 days, yeast will be harvested, washed and homogenized in a chloroform:methanol (1:1, v/v) and stored at -20C until further analysis. Total lipid extracts will be used for fatty acid methyl ester (FAME) preparation, which will be analysed by gas chromatography at IATS-CSIC. Expected outcomes and potential impact This proposal is highly ambitious and is expected to reinvigorate interests, and open new domains for inland pond aquaculture and imagining ponds as living labs for testing or validating restorative management. One impact factor publication that will be published open-access and with open-access data in a Q1 journal (according to Article Influence Score) will be published focused on the science of LC-PUFA mining and bio-machinery of land-locked European fishponds using low-trophic aquaculture and carp, while mitigating or reversing eutrophication. 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Versions External resources Indexed in OpenAIRE Communities Details DOI DOI Badge DOI 10.5281/zenodo.19701551 Markdown [](https://doi.org/10.5281/zenodo.19701551) reStructuredText .. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.19701551.svg :target: https://doi.org/10.5281/zenodo.19701551 HTML <a href="https://doi.org/10.5281/zenodo.19701551"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.19701551.svg" alt="DOI"></a> Image URL https://zenodo.org/badge/DOI/10.5281/zenodo.19701551.svg Target URL https://doi.org/10.5281/zenodo.19701551 Resource type Output management plan Publisher Zenodο Citation Export Technical metadata Created April 22, 2026 Modified April 22, 2026 Jump up About About Policies Infrastructure Principles Projects Roadmap Contact Blog Blog Support Help FAQ Developers REST API OAI-PMH Contribute GitHub Donate Funded by Powered by CERN Data Centre & InvenioRDM Status Privacy policy Cookie policy Terms of Use This site uses cookies. 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