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Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

Josiane Azizi et al.
HAL (France) · Papers
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This study reports the heterologous production, purification, and characterization of the cytochrome Cyc2 TAG1 cluster 1 (Cyc2 TAG1_1 ) from Ghiorsea bivora TAG1 in the outer membrane of Escherichia coli BL21. Using a specific gene construct we optimized solubilization and purification protocols to
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