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On the Origin of “Picket Fence” Peaks: Spectral Complexity, Mass Defect Uniformity, and Mass-to-Charge Ratio Alignment in Tandem Mass Spectra of Intact Proteins
biochemistrybiophysicscell-biologydevelopmental-biologygeneticsinfectious-diseasesinorganic-chemistryneuroscience
Spectra from tandem mass spectrometry (MS/MS) of intact proteins are often highly complex, containing numerous closely spaced peaks arising from fragment ions with similar m/z values. This high level of spectral density continues to challenge even state-of-the-art high-resolution mass
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