Precision Profiling of α‐1,4‐ Versus α‐1,6‐Glycosidase Activity Through Synthetic Linear and Branched Glycogen Tetramers Using Cyclic Ion Mobility Mass Spectrometry and High‐Performance Liquid Chromatography‐Mass Spectrometry

Accurately defining glycosidase specificity is often limited by access to well‐defined oligosaccharide probes and analytical tools capable of resolving regioisomers at low concentrations. We synthesized two linear and one branched glycogen‐mimetic tetrasaccharides, bearing exclusively 1,2‐ cis , nam