Flagship 54/90 Predefined two-factor interaction in a neuroimmune outcome is the preregistered question, not a demonstrated effect. CAUSAL STUDIES OF QIGONG, ACUPUNCTURE AND NEUROIMMUNE REGULATION AT THE LIMIT This 777-page Research Design Working Edition in the STARLI Acupuncture Trilogy, Family II, develops the research question of factorial attribution of neuroimmune assay changes to competing behavioral and procedural causes. Its core three-coupling is Qigong practice x acupuncture intervention x neuroimmune readouts. The scope is ethics-approved non-invasive observational datasets and ex-vivo assays; no new invasive intervention described. Ancient and contemporary philosophical terms are treated as research motivation and operational labels, not independent proof of physical forces or of treatment benefit. The candidate proposal asks whether predefined two-factor interaction in a neuroimmune outcome can be measured with transparent source identities and enough precision to distinguish the proposed account from ordinary exercise and generic procedure expectancy cause measured biomarker changes. A prospectively frozen evaluation would test blinded separation of breathing/movement effects from an acupuncture procedure label; stronger conventional comparisons include matched movement, rest, and appropriately authorized sham or simulation comparisons. Stop when orthogonal cell-safety evidence fails or factorial interaction does not survive controls, when calibration fails, or when a study lacks appropriate permission. Aborted and negative outcomes remain part of the full eligibility denominator. Thirty conditional mathematical research plates examine distinct modeling and verification roles with explicit units, assumptions and blind counterexamples. Eighty-two chapters contain 410 separately paginated investigations. Two hundred Research Gates frame distinct pairings of a target readout, orthogonal check, concealed challenge, rival account and refusal decision. Thirty-three Phoenix Answer Embryos propose future instruments, evidence contracts and cross-laboratory research tools. This content is prospective research design and contains no completed experimental outcomes. The source study contract is the STARLI 90 Flagship Master v1.3. Methodological ancestry supplied in that document includes DOIs 10.5281/zenodo.23480803, 10.5281/zenodo.23486020 and 10.5281/zenodo.23318840. These methods do not validate the new candidate; bibliography and individual formula suitability still require independent external checking. Full English PDF. Automated structure and lexical difference checks cannot establish scientific originality, causal validity or clinical efficacy. Author: Feng Cheng-en (33) x STARLI / Xingli Series: STARLI Acupuncture Trilogy / Flagship 54 of 90 Edition: Research Design Working Edition v1.0 / 2 November 2026 Join the STARLI Research Community Explore more than 100 interdisciplinary research themes and follow the evolving STARLI Research Gate community: https://zenodo.org/communities/starli-research-gate/

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