Transcriptomic Evidence Of Sympathetic Modulation Of Cells Involved In Peripheral Nerve Injury

Transcriptomic Evidence Of Sympathetic Modulation Of Cells Involved In Peripheral Nerve Injury | Zenodo Skip to main Communities My dashboard Log in Sign up Zenodo is currently experiencing slowness and intermittent outages due to heavy automated traffic from bots and AI crawlers. We are aware of the problem, and our team is focused on stabilizing the service. Thank you for your patience. 71st Annual Meeting Plastic Surgery Research Council - Los Angeles, California Published May 10, 2026 | Version v3 Conference paper Open Transcriptomic Evidence Of Sympathetic Modulation Of Cells Involved In Peripheral Nerve Injury Authors/Creators Ifeoluwa Shoyombo 1 Rahul Gorijavolu 1 Kaushik Madapati 1 Malcolm Brock 1 Sami Tuffaha 1 Fares Lebbos 1 Lee Dellon 1 Show affiliations 1.

1 Johns Hopkins Hospital, Baltimore, MD, USA, 2 University of Berkeley, Berkeley, CA, USA. Description PURPOSE: Peripheral nerve injury initiates complex regenerative programs involving Schwann cells, macrophages, fibroblasts, and endothelial cells. Sympathetic activity rises markedly after injury, yet its direct influence on the local repair microenvironment remains poorly defined. We hypothesized that nerve injury induces upregulation of adrenergic receptors across key cell types, enabling direct sympathetic modulation of immune and regenerative processes via catecholaminergic signaling. METHODS: We performed single-cell RNA sequencing (scRNA-seq) meta-analysis of two public datasets: (1) longitudinal mouse sciatic nerve crush injury (GEO: GSE198582) and (2) sympathetic/sensory ganglia transcriptomes (GSE175421). Using single-cell analysis, we profiled adrenergic receptor expression (α: ADRA1A/B/D, ADRA2A/B/C; Β: ADRB1/2/3) across cell types and timepoints (Days 0-14). Intercellular communication between sympathetic neurons and injury-site cells was inferred using NicheNet and CellPhoneDB v5. RESULTS: Adrenergic receptor expression was markedly increased following injury. ADRB2 (β₂-adrenergic receptor) exhibited a 3-5-fold rise, with receptor-positive Schwann cells and macrophages increasing from 15-20% at baseline to 60-75% at Day 3 post-injury. Temporal profiling revealed biphasic activation: early receptor induction in macrophages (Days 1-3) corresponding to inflammatory influx, followed by sustained Schwann cell expression (Days 7-14) during remyelination. Fibroblasts preferentially upregulated ADRA1B, while endothelial cells showed mixed α/β receptor expression. Ligand-receptor mapping identified sympathetic neuron catecholamine synthesis genes (TH, DBH, DDC) as key senders driving downstream injury-site transcriptional responses. CONCLUSION: Peripheral nerve injury elicits robust, time-dependent adrenergic receptor upregulation in multiple cell types, establishing a molecular framework for sympathetic regulation of both inflammatory and reparative phases. These findings reveal adrenergic signaling as a previously unrecognized determinant of nerve regeneration. Ongoing validation studies using denervation and pharmacologic blockade models aim to define optimal intervention windows for clinical translation. Notes Abstract ID: OS52 Files PSRC2026_OS52.txt Files (3.1 kB) Name Size Download all PSRC2026_OS52.txt md5:c4af761aa375c951da5a59fac1288e6f 3.1 kB Preview Download 69 Views 8 Downloads Show more details All versions This version Views Total views 69 35 Downloads Total downloads 8 4 Data volume Total data volume 25.0 kB 12.5 kB More info on how stats are collected.... Versions External resources Indexed in OpenAIRE Communities Keywords and subjects Keywords plastic surgery reconstructive surgery conference abstract PSRC 2026 Details DOI DOI Badge DOI 10.5281/zenodo.20101381 Markdown [](https://doi.org/10.5281/zenodo.20101381) reStructuredText .. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.20101381.svg :target: https://doi.org/10.5281/zenodo.20101381 HTML <a href="https://doi.org/10.5281/zenodo.20101381"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.20101381.svg" alt="DOI"></a> Image URL https://zenodo.org/badge/DOI/10.5281/zenodo.20101381.svg Target URL https://doi.org/10.5281/zenodo.20101381 Resource type Conference paper Publisher Zenodo Conference 71st Annual Meeting of the Plastic Surgery Research Council (PSRC 2026), Los Angeles, CA, USA, June 4-7, 2026 Rights License Creative Commons Attribution Non Commercial No Derivatives 4.0 International No further description. Read more Citation Export Technical metadata Created May 9, 2026 Modified May 9, 2026 Jump up About About Policies Infrastructure Principles Projects Roadmap Contact Blog Blog Support Help FAQ Developers REST API OAI-PMH Contribute GitHub Donate Funded by Powered by CERN Data Centre & InvenioRDM Status Privacy policy Cookie policy Terms of Use This site uses cookies. Find out more on how we use cookies Accept all cookies Accept only essential cookies

Related documents

• Sympathetic Nerve Activity During Hypoglycemia and Exercise#1172528
• Molecular Mediators of Nerve Injury Signaling#432833
• Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury#218250
• Proteomic Analysis in Paediatric Diabetes Type 1 (PAPD)#900658
• Cellular and molecular changes in the skin driving increased nociception and pain during burn injury and repair.#378527