Legionella stuttgartensis sp. nov. and Legionella nigrisilvae sp. nov., two new species isolated from a re-cooling plant and from a drinking water house installation in southern Germany.

Legionella stuttgartensis sp. nov. and Legionella nigrisilvae sp. nov., two new species isolated from a re-cooling plant and from a drinking water house installation in southern Germany - PMC Skip to main content An official website of the United States government Here's how you know Here's how you know Search Log in Primary site navigation Search Log in Search PMC Full-Text Archive Search in PMC Journal List User Guide As a library, NLM provides access to scientific literature. Inclusion in an NLM database does not imply endorsement of, or agreement with, the contents by NLM or the National Institutes of Health. Learn more: PMC Disclaimer | PMC Copyright Notice Int J Syst Evol Microbiol . 2026 Jun 2;76(6):007187. doi: 10.1099/ijsem.0.007187 Legionella stuttgartensis sp. nov. and Legionella nigrisilvae sp. nov., two new species isolated from a re-cooling plant and from a drinking water house installation in southern Germany Bibiana Rios Galicia 1, * , Patryk Krauze 1 , Caroline Hengerer 1 , Konstantin Licht 1 , Stefan Brockmann 1 , Jens Fleischer 1, * Author information Article notes Copyright and License information 1 Department of Health Protection, Infection Control and Epidemiology, Baden-Wuerttemberg Federal State Health Office, Ministry of Social Affairs, Health and Integration, Stuttgart, Germany * Bibiana Rios Galicia, bibiana.riosgalicia@sm.bwl.de * Jens Fleischer, Jens.Fleischer@sm.bwl.de The authors declare that there are no conflicts of interest. Supplement: Two supplementary figures and four supplementary tables are available with the online version of this article. Received 2025 Nov 18; Accepted 2026 May 15; Collection date 2026. Copyright © 2026 The Authors This is an open-access article distributed under the terms of the Creative Commons Attribution License. PMC Copyright notice PMCID: PMC13229132  PMID: 42227997 Abstract The genus Legionella encompasses environmental bacteria frequently present in water samples. Two strains of Legionella isolated from a re-cooling plant and from a drinking water house installation in southern Germany were identified as two different novel species. The isolates were both Gram-stain negative rods, negative to latex agglutination, able to grow at 37 °C on buffered charcoal–yeast extract agar with l -cysteine agar. The phenotypic characterization was complemented by whole-genome sequencing. The genomes of the strains WA2022007384 and WA2024007413 were sequenced and identified as two different novel species with a GC mol% content of 37.92 and 37.91, respectively. Both strains encoded antimicrobial resistance genes against beta-lactams, aminoglycosides and clusters for legioliulin biosynthesis that might confer fluorescence under UV light at 365 nm. The average nucleotide identity percentage between the strain WA2022007384 and its closest related strains was 87.41% to Legionella qingyii km489 T and 86.97% to Legionella gormanii NCTC11401 T . For the strain WA2024007413, the closest species were 98.28% to Legionella sp. PC997, 85.84% to L. gormanii NCTC11401 T and 85.61% to L. qingyii km489 T . The phylogenetic tree based on 164 concatenated core genes showed a separated clade neighbouring the strains L. gormanii NCTC11401 T and L. qingyii km489 T . These results confirm the description of the two novel species Legionella stuttgartensis sp. nov., with WA2022007384 T (=DSM 120481 T =NCCB 101094 T ) as its type strain, and Legionella nigrisilvae sp. nov., with WA2024007413 T (=DSM 120480 T =NCCB 101095 T ) as its type strain. Keywords: drinking water, drinking water house installation, Legionella nigrisilvae sp. nov., Legionella stuttgartensis sp. nov., new species, re-cooling plant, whole-genome sequencing (WGS) Introduction Species from the genus Legionella are Gram-negative aerobic rods found in sweet-water environments, water installations or in single-celled protozoa as natural hosts and reservoirs [ 1 ]. Some species are responsible for sporadic and epidemic outbreaks of atypical pneumonia, including the well-known legionellosis, Legionnaire’s disease caused mainly by Legionella pneumophila serogroup 1 (Lp1) [ 2 , 3 ]. The List of Prokaryotic names with Standing in Nomenclature (LPSN database accessed on 9 September 2025) recognizes 69 species of Legionella , 1 of which is described only as a metagenome assembly: Candidatus Legionella jeoni [ 4 ]. Despite being a diverse group, approximately half of the described species has been associated with respiratory human diseases [ 1 , 5 ]. They are often found in drinking water distribution systems such as showers, baths, heating systems or in re-cooling plants, cooling towers, air conditioning units and decorative water items, where aerosolization, dissemination and transmission can occur [ 6 , 7 ]. Due to their capability to form biofilms and replicate within eukaryotic cells like amoeba or alveolar macrophages, their survival rate to disinfectants is high and makes Legionella a large public health threat [ 6 , 8 ]. In Germany, the presence of Legionella in water installations is constantly monitored by the public health authorities responsible for the region administration, following the guidelines from the DIN EN ISO 19458:2006-12 (Water quality – Sampling for microbiological analysis, ISO 19458:2006). In this study, we present two Legionella strains isolated from a re-cooling plant and from a drinking water installation, which, based on genomic, phylogenetic and phenotypic analyses, represent two novel species within the genus Legionella . Methods Isolation and cultivation Water samples were collected from a re-cooling plant in Friolzheim and from a drinking water installation in Lahr, Germany, in October 2022 and September 2024, respectively. The samples were collected according to DIN EN ISO 19458:2006; Category b) by the Local Health Office from each region and analysed by the State Health Office of Baden-Württemberg. The isolation of Legionella species was performed by a standard culture technique on glycine–vancomycin–polymyxin B–cycloheximide (GVPC) agar medium following DIN EN ISO 11731:2019-03. Fifty millilitres of the water sample was filtered through a membrane filter (0.45 µm). After filtration, the membrane filters were coated with an acid buffer solution (0.2 M HCl/KCl, pH 2.0–2.2) and left to act for 5 min. The buffer was aspirated and rinsed with a Ringer’s nutrient solution (1:10). The filters were placed on a GVPC agar plate and incubated, and growth was monitored after 5, 7 and 10 days of incubation. Presumptive Legionella colonies were sub-cultured on buffered charcoal yeast extract agar (BCYE) with cysteine and in tryptone soya agar with 5% sheep blood agar medium (Thermo Fisher Scientific Diagnostic). Cultivation and sub-cultivation were performed aerobically at 36±1 °C under an atmosphere of 95% relative humidity. Isolates were preserved at −80±2 °C in Cryobank tubes with Cryobeads following the manufacturers’ instructions. Identification and genome sequencing The sub-cultures that did not grow in blood agar medium, considered suspicious to be Legionella isolates, were tested using the Oxoid Legionella Latex Test (Part No. DR0800M, Thermo Fisher Scientific Diagnostic), a qualitative latex slide agglutination assay used for the identification of some serogroups of Legionella species: Legionella longbeachae serogroup 1 and 2, Legionella bozemanii serogroup 1 and 2, Legionella dumoffii , Legionella gormanii , Legionella jordanis , Legionella micdadei and Legionella anisa. Legionella -suspected isolates were identified with the matrix-assisted laser desorption ionization time-of-flight MS technique with the VITEK MS PRIME system (bioMerieux) following the manufacturer’s instruction. DNA extraction was performed following a protocol for preparation of genomic DNA using mechanical lysis [ 9 ]. Briefly, cells were grown and resuspended in PBS to a density of 1.0 (A600nm). The cell pellet was suspended on 0.15 ml of PBS buffer and incubated at 99 °C for 5 min, followed by a mechanical lysis using acid-washed glass beads (425–600 µm) and a cell disruptor (frequency: 30 Hz) for 10 min. The tubes were then centrifuged at 12,000 g for 5 min. The supernatant was recovered and diluted in 100 µl of nuclease-free water and quantified using fluorometric quantification (Qubit, Thermo Fisher Scientific). Legionella -suspected isolates were analysed by amplifying the 16S rRNA gene sequences using the universal primers 27F and 1492R [ 10 ]. Amplicons were Sanger sequenced and aligned with sequences from Legionella -related species at the non-redundant GenBank 16S ribosomal RNA database from the National Center for Biotechnology Information (NCBI). Phylogenetic trees were generated based on maximum-likelihood using Unipro UGENE software (version 53.0) and iTOL (version 6.5.8) [ 11 , 12 ]. Whole-genome sequencing was performed using the library preparation of MiSeq DNA library prep kit from Illumina and sequenced using a 150 bp paired-end run on an Illumina MiSeq platform (Illumina). Reads were quality-controlled, decontaminated from sequencing artefacts or adapters and merged using BBtools (version 37.62) [ 13 ]. Assembly was performed using SPAdes (version 3.15.0) [ 14 ] under a high-coverage isolate tag for filtered and merged reads. The quality of the assembled genome was assessed using QUAST (version 5.3.0) [ 15 ], and completeness and contamination levels were further verified using CheckM2 (version 1.2.2) ( https://github.com/Ecogenomics/CheckM ). The genome assemblies and raw files were submitted to the European Nucleotide Archive (ENA) database under the BioProject number PRJEB90345. Genome annotation and gene prediction was done with Prokka (version 1.14.5) [ 16 ]. Secondary metabolite biosynthetic gene clusters were analysed with antiSMASH 8.0 database [ 17 ]. Physiology and chemotaxonomy Strains were analysed under an automated system for characterization of biochemical features using Vitek 2 Compact System version 9.04.3 (bioMerieux). The test panels contained 47 colorimetric tests that included the detection of aminopeptidases, decarboxylase tests (for ornithine and arginine) and additional miscellaneous tests (urease, beta-glucuronidase, Ala-Phe-Pro acrylamidase or Pyrrolidonyl Arylamidase). The card was sealed and inserted into the VITEK 2 reader-incubator module (35.5 °C incubation temperature) and subjected to a kinetic fluorescence measurement every 15 min. The results were interpreted by the ID-BCL database. Cellular fatty acid (CFA) determination was performed by the DSMZ services Leibniz-Institut DSMZ–Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany). A biomass pellet of the strains WA2022007384 T and WA2024007413 T was collected from BYCE agar plates of 72 h incubation, frozen and sent for analysis to the DSMZ services, following the standard protocol recommended for the MIDI Microbial Identification System (MIDI Inc., version 6.1, Newark, DE). The composition of CFAs was identified using the Sherlock microbial identification system (TSBA40 library; MIDI Inc.). Taxonomy analysis Genomes were taxonomically classified with GTDB-Tk (version 2.1.0) [ 18 , 19 ] with the database GTDB (R220) [ 20 ]. Genome contigs were submitted to the Type (Strain) Genome Server (TYGS), for a whole genome-based taxonomic analysis confirmation [ 21 ] using the fast distance-based phylogeny inference programme FastME 2.0 [ 22 ]. Since the isolates were not assigned to a reliable taxonomic identity, they were further analysed using genomic comparison to all members of the genus Legionella . Genomes of all species of Legionella were collected using the NCBI genome browser ( https://www.ncbi.nlm.nih.gov/datasets/genome/ ) considering type strains according to the LPSN. Phylogenetic trees were constructed based on 164 concatenated core genes using the concatenated-gene-alignment-fasta tool from Anvi’o version 8.1 [ 23 ], inferred by maximum-likelihood using FastTree version 2.1.11 [ 24 ]. The generated trees were visualized with iTOL (version 6.5.8) [ 12 ] and annotated with the tool table2itol in R studio version 3.6.1 ( https://github.com/mgoeker/table2itol ). The identity parameters for taxonomic delineation, digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI), were calculated using the Genome-to-Genome Distance Calculator (GGDC version 3.0) ( https://ggdc.dsmz.de/ ) from the DSMZ [ 25 ] and the software FastANI version 1.33 ( https://github.com/ParBLiSS/FastANI ), respectively. Accession numbers of the reference genomes used in the analysis are provided in Table S1 (available in the online Supplementary Material). Results and discussion The strains described in this work were isolated within a routine detection of Legionella sp. from a re-cooling plant and from a drinking water installation, performed by the Local Health Office of Friolzheim and Lahr/Schwarzwald, Germany, and analysed by the Health Office of the state of Baden-Württemberg. The strain WA2022007384 was isolated from a water sample taken in October 2022, and the strain WA2024007413 was obtained from a water sample taken in September 2024. The routine identification using MS (Vitek MS Prime, bioMerieux) did not provide an identity match as for other common species of Legionella , L. pneumophila or L. anisa , and depicted a similarity match of 96% to Pasteurella cannis . However, the differential test of growth in blood agar resulted negative for both strains. The 16S rRNA sequences of the strain WA2022007384 showed a 100% identity to Legionella sp. L-29 and 99.41% to Legionella sp. PC997, two strains not assigned to a species. In contrast, the sequence of WA2024007413 exhibited 99.92% sequence identity to Legionella sp. PC997 and 99.2% to Legionella resiliens 8cVS16 ( Fig. 1 ). Fig. 1. Maximum-likelihood tree showing the relationship between strains WA2022007384 T and WA2024007413 T to species of Legionella based on a 16S rRNA gene alignment (1,400 bp) using Klebsiella pneumoniae BK-RB-17 as an outgroup, created with Unipro UGENE software. Strains of novel species are highlighted in blue. Branch lengths correspond to sequence differences indicated by the scale bar. GenBank accession numbers and strain names are given for each leaf. *The 16S sequence of the strain PC997 and PC1000 were retrieved from the genome sequence using ContEst16S [ 37 ]. Open in a new tab Genomic features The genome of WA2022007384 was assembled in 59 contigs and encompassed 4,334,595 bp with a 37.92 GC mol%. The number of coding DNA sequences (CDS) was 3,807, including 3 rRNA and 42 tRNA genes. The genome metrics of both strains lay within the ranges of the genus Legionella and approach similar ranges within the neighbour strains according to the phylogram ( Fig. 2 ). The genome assembly of the strain WA2024007413 included 26 contigs and 3,881,291 bp. The GC mol% was 37.91 and the CDS of 3434, including 3 rRNA and 44 tRNA genes. The N50 of both assemblies was 465 and 817, respectively. Fig. 2. Maximum-likelihood phylogenomic tree of Legionella based on 164 concatenated core genes, with L. stuttgartensis and L. nigrisilvae as novel species, rooted with Klebsiella pneumoniae ATCC 13883T. Strains of novel species WA2022007384 T and WA2024007413 T and are highlighted in blue. Bootstrap values (1,000 replicates) are depicted above branches, and branch lengths correspond to sequence differences indicated by the scale bar. Open in a new tab The genome of both species encoded the gene resistance cluster blaFEZ-1 coding for a metallo- β -lactamase operon, already reported in other species of Legionella . It might confer resistance against cephalosporin, penicillin and carbapenem when expressed [ 26 ]. Additionally, the strain WA2024007413 encoded two more antibiotic resistance clusters: an aminoglycoside phosphotransferase APH(9)-Ia and the β -lactamase OXA-29 that will confer resistance to aminoglycosides and penicillin-like antibiotics, respectively. All resistance gene clusters identified among the two species have been described in L. gormanii [ 27 ] and L. pneumophila [ 28 ]. Phylogenomic analysis The phylogenetic analysis based on 16S rRNA gene ( Fig. 1 ) and the phylogenomic analysis based on 164 concatenated core genes ( Figs 2 and S1) confirmed that the strains WA2022007384 and WA2024007413 belong to a single clade neighbouring species of Legionella qingyii [ 29 ] and L. gormanii [ 30 ]. The strain WA2022007384 exhibited 87.41% ANI to L. qingyii km489 T and 86.97% to L. gormanii NCTC11401 T , while the strain WA2024007413 presented 98.28% ANI to Legionella sp. PC997, 85.84% to L. gormanii NCTC11401 T and 85.61% to L. qingyii km489 T . The high identity ANI percentage to the strain PC997 of Legionella sp. [ 31 ] lies within the intra-species threshold of >95% [ 32 ]. The genome of the strain PC997 has been reported as an environmental isolate of Legionella . However, it has not been validly described as a novel species [ 31 ]. The high ANI percentage of the strain PC997 to our isolate WA2024007413 and the topology observed in the phylogenetic tree suggest that both isolates belong to the same species: Legionella nigrisilvae sp. nov. Despite the similarities among the strains WA2022007384 and WA2024007413, the ANI percentage stayed within the inter-species threshold with 86.81%, confirming the description of two different species of Legionella ( Fig. 3 , Table S2). Fig. 3. Correlation matrix of average amino acid identity scores (ANI) calculated among reference species closely related to the strains WA2022007384 T and WA2024007413 T . ANI percentage corresponds to colour indicated by the scale bar on the right side. Open in a new tab The in silico GGDC estimated the strain WA2022007384 to have a 51.70% dDDH similarity to the genome of L. gormanii NCTC11401 T , 48.00% to Legionella qingyii km489 T and 55.30% to the strain Legionella sp. PC997, with a distance of 0.3220, 0.2985 and 0.2699, respectively. The strain WA2024007413 presented a dDDH similarity of 51.70% to the genome of L. gormanii NCTC11401 T , 46.50% to Legionella qingyii km489 T and 90.80% to the strain Legionella sp. PC997, with a respective distance of 0.2943, 0.3338 and 0.0764. A distance bigger than 0.258 was considered as a threshold for species delineation [ 25 ]. Physiology, cell morphology and chemotaxonomy The subculture of both strains was performed on BCYE-Cys agar plates with activated charcoal. Bacterial colonies were visible within 5 to 10 days as white convex colonies of defined entire edges of 2 to 5 mm in diameter of moist shiny appearance and fluorescent under UV light at 365 nm (Fig. S2). Both strains resulted negative to the Gram stain test and present thin small rods of ~0.3 to 0.5 µm wide and 5 to 10 µm long. The strain WA2024007413 presented a microscopic morphology of rods arranged in long chains, while the strain WA2022007384 showed an aleatory rod distribution. Both strains produced colonies that showed a light blue colour under UV light at 365 nm and blue fluorescence in darkness under UV light at 365 nm (Fig. S2). Fluorescent emission is common in some species of environmental Legionella and has been proposed as a confirmation method for detecting Legionella colonies isolated from water samples [ 33 ]. The genes responsible for the colony’s fluorescence lgl (legioliulin) were annotated within the secondary metabolism annotation, these biosynthetic clusters might be involved in the biosynthesis of a natural fluorophore [ 34 ]. Legioliulin genes were encoded within both genomes ( Fig. 4 ) and might be responsible for the production of fluorescence under UV light at 365 nm. Fig. 4. Gene cluster distribution of legioliulin genes, potentially responsible for the blue fluorescence observed under UV light at 365 nm, encoded in Legionella stuttgartensis WA2022007384 T and L. nigrisilvae WA2024007413 T . The annotation files are available under the accessions GCA_977063405.2 and GCA_977063395.2. Open in a new tab The annotation files are available under the accessions GCA_977063405.2 and GCA_977063395.2. The agglutination test resulted negative for both strains, and none of the strains grew in blood agar as a differential test. The panel of biochemical parameters analysed by Vitek 2 (bioMerieux) resulted in a negative expression of aminopeptidases, carbohydrate utilization and decarboxylase tests. However, both strains tested positive for the expression of phosphatase activity (Table S3). Major CFAs detected were branch-chain acids. The strain WA2022007384 T presented 34.2% of saturated anteiso-C 15 : 0 , 18.8% of iso-C 16 : 0 , 12% of C 16:1 ω 7c/C 16:1 ω 6c, 6.7% of anteiso-C 17:0 and 6.3% of iso-C 14 : 0 . The strain WA2024007413 T showed 30.8% of saturated anteiso-C 15 : 0 , 26.2% of iso-C 16:0 , 11.5% of anteiso-C 17 :0 and 8.3% of C 16:1 ω 7c/C 16:1 ω 6c. The fatty acid profile showed a very similar spectrum to that of the genus Legionella , with dominating a15:0, i16:0 and a17:0 [ 35 ]. The major fatty acids of WA2022007384 T and WA2024007413 T have a similar pattern percentage of fatty acids to species of L. gormanii , L. dumoffii and L. qingyii [ 36 ], with a distinctive difference of presenting a higher percentage of C 16:1 ω 7c/C 16:1 ω 6c than other Legionella species. Table 1 shows the CFA profile content of strains that neighbour the strains WA2022007384 T and WA2024007413 T within the same clade. Additional fatty acid profile of other Legionella species ( L. dumoffii ATCC 33279 T , L. anisa ATCC 35292 T , Legionella parisiensis ATCC 35299 T , Legionella bozemanae ATCC 33217 T , Legionella tucsonensis ATCC 49180 T and L. resiliens 8cVS16 T ) is shown in Table S4. Table 1. CFA compositions of strains WA2022007384 T , WA2024007413 T , L. qingyii km488 T and L. gormanii ATCC 33297 T . Fatty acid WA2022007384 T DSM 120481 WA2024007413 T DSM 120480 L. qingyii km488 T L. gormanii ATCC 33297 T Saturated straight-chain C 14 : 0 0.4 2.0 – C 15 : 0 3.9 0.6 3.3 2.3 C 16 : 0 3.5 1.6 8.3 9.6 C 17 : 0 1.8 1.0 4.8 3.6 C 18 : 0 0.6 0.5 1.9 1.5 C 19 :0 0.4 0.4 – – C 20 : 0 0.3 0.7 – 0.7 Saturated branched-chain Anteiso-C 11 : 0 0.2 Anteiso-C 13 : 0 0.2 Iso-C 14 : 0 6.3 4.8 2.9 4.3 Iso-C 15 : 0 0.9 0.6 – – Anteiso-C 15 : 0 34.2 30.8 25.9 22.1 Iso-C 16 : 0 18.8 26.2 14.5 19.4 Iso-C 17 : 0 0.5 0.5 – 0.7 Anteiso-C 17 : 0 6.7 11.5 13.8 9.9 Iso-C 18 : 0 0.2 0.8 Anteiso-C 19 : 0 0.3 Iso-C 20 : 0 0.2 Branched-chain hydroxy C 13 :0 iso 3OH 0.2 C 14:0 iso 3OH C 16:0 iso 3OH 0.2 0.2 Unsaturated/hydroxy C 14 : 1 ω 5c 0.2 0.2 C 15 : 1 ω 4c 1.0 C 15 : 1 ω 6c 3.0 1.3 1.8 2.1 C 16 : 1 ω 5c 1.5 0.8 – – C 16 : 1 ω7 c C 16 : 1 ω 11c C 17 : 0 cyclo 1.1 7.4 4.8 9.6 C 18 : 1 ω 7c – – Summed features 2 (C 16 : 1 iso I/C 14:0 3OH) 0.3 0.2 (C 16:1 ω 7c/C 16:1 ω 6c) 12 8.3 (C 16:1 ω 6c/C 16:1 ω 7c) 0.3 (C 18:1 ω 7c/C 18:1 ω 6c) 0.4 Open in a new tab (-) Not available Data is marked with a middle hyphen. Protologue Description of Legionella stuttgartensis sp. nov. Legionella stuttgartensis (N.L. fem. adj. stuttgartensis , pertaining to Stuttgart). This species was isolated in Stuttgart by the Public Health department of the state Baden-Württemberg from a re-cooling tower in the municipality of Friolzheim. Legionella stuttgartensis is a Gram-negative, oxidase- and catalase-positive aerobic rod-shaped bacterium. It produces white colonies at 35–37 °C on BCYE medium with cysteine. The type strain genome of Legionella stuttgartensis WA2022007384 T (=DSM 120481 T =NCCB 101094 T ) has a G+C content of 37.92 mol% and encodes 3,807 genes. Major CFAs are anteiso-C 15 : 0 , iso-C 16 : 0 , C 16:1 ω 7c/C 16:1 ω 6c, anteiso-C 17 : 0 and iso-C 14 : 0 . The 16S rRNA gene GenBank accession number of strain WA2022007384 T is PV778058 . The GenBank accession number for the whole-genome shotgun project is GCA977063405. Description of Legionella nigrisilvae sp. nov. Legionella nigrisilvae (L. masc. adj. niger , black; L. fem. n. silva , forest; N.L. gen. n. nigrisilvae , of the Black Forest). Gram-negative, oxidase- and catalase-positive aerobic rod-shaped bacterium species isolated in Stuttgart by the Public Health department of the state Baden-Württemberg from a drinking water installation taken in the district of Lahr/Schwarzwald (Black Forest) in Germany. L. nigrisilvae is a Gram-negative stain, oxidase and catalase-positive aerobic rod-shaped bacterium. It produces white colonies after 5 to 10 days of incubation at 35–37 °C on BCYE medium with cysteine. The type strain genome of L. nigrisilvae WA2024007413 T (=DSM 120480 T =NCCB 101095 T ) has a G+C content of 37.91 mol% and encodes 3,434 genes. Major CFAs are anteiso-C 15 : 0 , iso-C 16:0 , anteiso-C 17 : 0 and C 16:1 ω 7c/C 16:1 ω 6c. The 16S rRNA gene GenBank accession number of strain WA2024007413 T is PV778059 . The GenBank accession number for the whole-genome shotgun project is GCA 977063395. Supplementary material Supplementary Material 1. ijsem-76-07187-s001.pdf (367.4KB, pdf) DOI: 10.1099/ijsem.0.007187 Supplementary Material 2. ijsem-76-07187-s002.xlsx (42.2KB, xlsx) DOI: 10.1099/ijsem.0.007187 Acknowledgements The authors sincerely appreciate all contributions made by the members of the LGA-Labor, Water Hygiene and Bacteriology Laboratories, as well as the Health Authorities in Enzkreis and Ortenaukreis, Germany. They extend their gratitude to the curators of the German Bacterial Collection (DSMZ) and the Netherlands Culture Collection of Bacteria (NCCB) for their guidance and support with the strain deposit. Abbreviations ANI average nucleotide identity ANI average nucleotide identity BCYE buffered charcoal yeast extract agar CFA cellular fatty acid Cys- without l-cysteine Cys+ with l-cysteine dDDH digital DNA–DNA hybridization GGDC Genome-to-Genome Distance Calculator GVPC glycine–vancomycin–polymyxin B–cycloheximide agar medium LPSN List of Prokaryotic Names with Standing in Nomenclature NCBI National Center for Biotechnology Information Footnotes Funding: The authors state that there is no funding information. Accession No: The GenBank accession numbers for the 16S rRNA genes of strains WA2022007384 and WA2024007413 are PV778058 and PV778059 , respectively. The GenBank accession numbers for the whole genome sequences are GCA977063405 and GCA977063395, respectively. Contributor Information Bibiana Rios Galicia, Email: bibiana.riosgalicia@sm.bwl.de. Patryk Krauze, Email: patryk.krauze@sm.bwl.de. Caroline Hengerer, Email: caroline.hengerer@sm.bwl.de. 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Supplementary Materials Supplementary Material 1. ijsem-76-07187-s001.pdf (367.4KB, pdf) DOI: 10.1099/ijsem.0.007187 Supplementary Material 2. ijsem-76-07187-s002.xlsx (42.2KB, xlsx) DOI: 10.1099/ijsem.0.007187 Articles from International Journal of Systematic and Evolutionary Microbiology are provided here courtesy of Society for General Microbiology and the International Union of Microbiological Societies ACTIONS View on publisher site PDF (2.7 MB) Cite Collections Permalink RESOURCES Similar articles Cited by other articles Links to NCBI Databases Follow NCBI NCBI on X (formerly known as Twitter) NCBI on Facebook NCBI on LinkedIn NCBI on GitHub NCBI RSS feed Connect with NLM NLM on X (formerly known as Twitter) NLM on Facebook NLM on YouTube National Library of Medicine 8600 Rockville Pike Bethesda, MD 20894 Web Policies FOIA HHS Vulnerability Disclosure Help Accessibility Careers NLM NIH HHS USA.gov Back to Top

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