Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress

Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress | bioRxiv Skip to main content Home About Submit ALERTS / RSS Search for this keyword Advanced Search New Results Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress Arthur Poitout , José M. Ugalde , Lucille Gorgues , Armelle Dongois , Patricia Scholz , Philippe Nacry , Carine Alcon , Jean-Bernard Fiche , Xavier Dumont , Marcelo Nollman , Komal Jhala , Anton Schäffner , Yvon Jaillais , Lionel Verdoucq , View ORCID Profile Andreas J. Meyer , Alexandre Martinière doi: https://doi.org/10.64898/2026.01.19.698185 Arthur Poitout 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site José M. Ugalde 2 INRES-Chemical Signalling, University of Bonn , Römerstraße 164, 53117 Bonn, Germany Find this author on Google Scholar Find this author on PubMed Search for this author on this site Lucille Gorgues 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Armelle Dongois 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Patricia Scholz 3 Laboratoire Reproduction et Développement des Plantes, Université de Lyon , ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Philippe Nacry 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Carine Alcon 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Jean-Bernard Fiche 4 Centre de Biochimie Structurale, Centre National de la Recherche Scientifique Unité Mixte de Recherche 5048, Institut National de la Santé et de la Recherche Médicale U1054, Université de Montpellier, 34090 Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Xavier Dumont 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Marcelo Nollman 4 Centre de Biochimie Structurale, Centre National de la Recherche Scientifique Unité Mixte de Recherche 5048, Institut National de la Santé et de la Recherche Médicale U1054, Université de Montpellier, 34090 Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Komal Jhala 5 Department of Environmental Sciences, Institute of Biochemical Plant Pathology , Helmholtz Zentrum München, Neuherberg, Germany Find this author on Google Scholar Find this author on PubMed Search for this author on this site Anton Schäffner 5 Department of Environmental Sciences, Institute of Biochemical Plant Pathology , Helmholtz Zentrum München, Neuherberg, Germany Find this author on Google Scholar Find this author on PubMed Search for this author on this site Yvon Jaillais 3 Laboratoire Reproduction et Développement des Plantes, Université de Lyon , ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Lionel Verdoucq 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site Andreas J. Meyer 2 INRES-Chemical Signalling, University of Bonn , Römerstraße 164, 53117 Bonn, Germany Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Andreas J. Meyer Alexandre Martinière 1 IPSiM, Univ Montpellier, CNRS, INRAE, Institut Agro , Montpellier, France Find this author on Google Scholar Find this author on PubMed Search for this author on this site For correspondence: alexandre.martiniere{at}cnrs.fr Abstract Full Text Info/History Metrics Supplementary material Preview PDF ABSTRACT The spatiotemporal organization of proteins and lipids within membranes is crucial for ensuring proper cellular signaling. While the segregation of proteins and lipids into membrane nanodomains is well established, it remains unclear whether nanodomains can generate gradients of small diffusible molecules. In plants, reactive oxygen species (ROS), especially hydrogen peroxide (H 2 O 2 ), act as a key signaling molecules in response to environmental stimuli such as osmotic stress. However, how extracellular H 2 O 2 affects intracellular signaling has remained unknown. Here, we show that osmotic stimulation induces the formation of localized, H 2 O 2 -rich nano-environments at the cytoplasmic face of the plasma membrane (PM) in Arabidopsis root cells. Using a PM-tethered H 2 O 2 biosensor, we found that these oxidized nanodomains arise from the clustering of RESPIRATORY BURST OXIDASE HOMOLOGs (RBOHs) and RHO OF PLANTS 6 (ROP6), in coordination with aquaporin-mediated H 2 O 2 transport via the PLASMA MEMBRANE INTRINSIC PROTEIN2;7 (PIP2;7). These local redox hotspots at the PM create a feedforward loop in which H 2 O 2 enhances ROP6 nanoclustering thereby amplifying ROS signaling. Disruption of H 2 O 2 production or transport dampens both ROP6 clustering and anisotropic cell expansion, indicating a crucial role for spatially-confined redox signaling in regulating plant growth under osmotic stress. Our findings propose a model in which ROP6/RBOHD-F/PIP2;7 nanodomains function as discrete redox signaling units, redefining ROS signaling at the PM as a structured, signal-specific, and compartmentalized process. Competing Interest Statement The authors have declared no competing interest. Funder Information Declared Agence Nationale de la Recherche , ANR-10-INBS-04 , ANR-19-CE20-0008 , ANR-24-CE92-0024 Deutsche Forschungsgemeinschaft , DFG 545516367 European Research Council , 101001097-LIPIDEV European Molecular Biology Organization, https://ror.org/04wfr2810 , ALTF 750-2023 Marie Curie Sklodowska Action , HORIZON-TMA-MSCA-PF-EF No 101151872-SONA Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license . Back to top Previous Next Posted January 21, 2026. Download PDF Supplementary Material Email Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. You are going to email the following Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress Message Subject (Your Name) has forwarded a page to you from bioRxiv Message Body (Your Name) thought you would like to see this page from the bioRxiv website. Your Personal Message CAPTCHA This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. Share Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress Arthur Poitout , José M. Ugalde , Lucille Gorgues , Armelle Dongois , Patricia Scholz , Philippe Nacry , Carine Alcon , Jean-Bernard Fiche , Xavier Dumont , Marcelo Nollman , Komal Jhala , Anton Schäffner , Yvon Jaillais , Lionel Verdoucq , Andreas J. Meyer , Alexandre Martinière bioRxiv 2026.01.19.698185; doi: https://doi.org/10.64898/2026.01.19.698185 Share This Article: Copy Citation Tools Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress Arthur Poitout , José M. Ugalde , Lucille Gorgues , Armelle Dongois , Patricia Scholz , Philippe Nacry , Carine Alcon , Jean-Bernard Fiche , Xavier Dumont , Marcelo Nollman , Komal Jhala , Anton Schäffner , Yvon Jaillais , Lionel Verdoucq , Andreas J. Meyer , Alexandre Martinière bioRxiv 2026.01.19.698185; doi: https://doi.org/10.64898/2026.01.19.698185 Citation Manager Formats BibTeX Bookends EasyBib EndNote (tagged) EndNote 8 (xml) Medlars Mendeley Papers RefWorks Tagged Ref Manager RIS Zotero Tweet Widget Facebook Like Google Plus One Subject Area Cell Biology Subject Areas All Articles Animal Behavior and Cognition (8164) Biochemistry (19027) Bioengineering (15171) Bioinformatics (45543) Biophysics (22958) Cancer Biology (20187) Cell Biology (27403) Clinical Trials (138) Developmental Biology (14189) Ecology (21468) Epidemiology (2067) Evolutionary Biology (25877) Genetics (16388) Genomics (23907) Immunology (19092) Microbiology (43353) Molecular Biology (18423) Neuroscience (95468) Paleontology (715) Pathology (3045) Pharmacology and Toxicology (5222) Physiology (8304) Plant Biology (16326) Scientific Communication and Education (2119) Synthetic Biology (4663) Systems Biology (10422) Zoology (2432)

Related documents

• Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress#308230
• A review of flavonoids at the crossroads of plant defense: integrating biotic and abiotic stress tolerance through AI- and CRISPR/Cas-guided metabolic reprogramming.#457394
• Plant BTB (Broad-Complex, Tramtrack, and Bric-à-Brac) Proteins: Structural Features, Biological Functions, and Roles in Stress Responses.#12860
• A New Role for Plastid Thioredoxins in Seed Physiology in Relation to Hormone Regulation#1146603
• Plant Stanols and Gene Expression Profile#1096171