Site-specific bio-efficacy of Interceptor G2 and Royal Guard against highly pyrethroid- 1 resistant Anopheles gambiae s.l. in experimental hut in southwestern Burkina Faso

Site-specific bio-efficacy of Interceptor G2 and Royal Guard against highly pyrethroid- 1 resistant Anopheles gambiae s.l. in experimental hut in southwestern Burkina Faso | Research Square Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Article Site-specific bio-efficacy of Interceptor G2 and Royal Guard against highly pyrethroid- 1 resistant Anopheles gambiae s.l. in experimental hut in southwestern Burkina Faso Antoine SANOU, Moussa Wandaogo GUELBEOGO, Madou TAPSOBA, Benoît SANON, and 13 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9258699/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 4 You are reading this latest preprint version Abstract Insecticide-treated nets (ITNs) lowered malaria cases and deaths by 68% in endemic regions by 2015, but rising resistance has slowed progress. Burkina Faso faces high resistance levels challenging vector control. This study assessed the effectiveness of Interceptor G2 (IG2) and Royal Guard (RG) ITNs against highly resistant (< 10% mortality rate to alpha-cypermethrin 0.05%) Anopheles gambiae s.l. in Experimental Hut Trials in Tannounyan region, Burkina Faso. Among 22,234 collected mosquitoes collected over three years, 66.6% were An. gambiae s.l. with Anopheles coluzzii and An. gambiae s.s., dominant in Tengrela and Tiefora respectively. Unwashed IG2 ITNs induced the highest mosquito mortality rates, 57% and 34% as 72-hour mortality in Tiefora and Tengrela respectively; mortality rates were higher for An. gambiae than An. coluzzii and significantly higher in both sites compared to standard ITNs. Net washing or aging reduced the efficacy of both IG2 and RG. Only unwashed RG nets reduced blood feeding success compared to standard ITNs. Follow up on the sublethal effects of the different arms showed, RG ITNs drastically reduced Anopheles gambiae s.l. offspring number compared to other nets. Results suggest, dual-ingredient nets' impact varies with local vector ecology, underscoring the need for targeted strategies of deployment and monitoring. Health sciences/Diseases Biological sciences/Ecology Earth and environmental sciences/Ecology Biological sciences/Zoology Anopheles gambiae s.l. Interceptor G2 Royal Guard experimental hut Burkina Faso insecticide resistance sublethal effect. Full Text Additional Declarations No competing interests reported. Supplementary Files SanouetAlSupplementaryTablesV1.5.217032026.pdf Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Revision requested 03 Apr, 2026 Editor assigned by journal 31 Mar, 2026 Submission checks completed at journal 31 Mar, 2026 First submitted to journal 29 Mar, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information

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