Three-Dimensional Volumetric Reconstruction of Native Chilean Pollen via Lens-Free Digital In-line Holographic Microscopy

[2601.14205] Three-Dimensional Volumetric Reconstruction of Native Chilean Pollen via Lens-Free Digital In-line Holographic Microscopy Skip to main content Search Submit Donate Log in Physics > Optics arXiv:2601.14205 (physics) [Submitted on 20 Jan 2026 ( v1 ), last revised 27 Aug 2026 (this version, v3)] Title: Three-Dimensional Volumetric Reconstruction of Native Chilean Pollen via Lens-Free Digital In-line Holographic Microscopy Authors: J. Staforelli-Vivanco , V. Salamanca-Levi , B. Muñoz-Cepeda , R. Jofré-Cerda , C. Valdés-Cruces , M. Rondanelli-Reyes , I. Lamas , J. M. Troncoso View PDF HTML (experimental) Abstract: This study presents a methodology for the three-dimensional volumetric reconstruction and morphological characterization of native Chilean pollen grains using lens-free Digital In-line Holographic Microscopy (DLHM). A 532 nm point source, a 3.45 $\mu$m pixel pitch CMOS sensor and a source--sample distance of $0.46$ mm with a $25$ mm stand-off give a magnification of $\simeq 55\times$ and an effective pixel of 62.9 nm. Wavefronts of \textit{Anthemis cotula}, \textit{Gevuina avellana} and \textit{Conium maculatum} were reconstructed by the Kirchhoff-Helmholtz transform, yielding volumes from $3780.2 \pm 18$ to $4320.5 \pm 15$ $\mu$m$^3$ and sphericities from $0.76 \pm 0.03$ for the echinate \textit{A. cotula} to $0.89 \pm 0.02$ for \textit{G. avellana}. Three independent targets make these reconstructions traceable. Certified polystyrene microspheres ($\varnothing = 20.0$ $\mu$m) make the source--sample distance an optically retrieved quantity, the 2.5D height map returning $2068$ $\mu$m$^3$ against an analytical $2094.4$ $\mu$m$^3$. A human erythrocyte, reconstructed with a haemoglobin refractive-index increment taken from the literature rather than fitted, returns $88.9$ fL with a $2.36$ $\mu$m rim and a $1.03$ $\mu$m dimple, within physiological range. Finally, transport-of-intensity retrieval on bright-field micrographs from a separate, independently calibrated instrument reproduces the projected morphology of \textit{G. avellana}, though no volume follows from it. These results support label-free "digital fingerprints" for automated melissopalynology in a South American biodiversity hotspot. Comments: 11 pages, pre-print article Subjects: Optics (physics.optics) ; Biological Physics (physics.bio-ph); Quantitative Methods (q-bio.QM) Cite as: arXiv:2601.14205 [physics.optics] (or arXiv:2601.14205v3 [physics.optics] for this version) https://doi.org/10.48550/arXiv.2601.14205 Focus to learn more arXiv-issued DOI via DataCite Submission history From: Juan Staforelli-Vivanco [ view email ] [v1] Tue, 20 Jan 2026 18:07:32 UTC (2,795 KB) [v2] Mon, 9 Mar 2026 16:46:09 UTC (2,797 KB) [v3] Thu, 27 Aug 2026 23:50:57 UTC (5,542 KB) Full-text links: Access Paper: View PDF HTML (experimental) TeX Source view license Current browse context: physics.optics < prev | next > new | recent | 2026-01 Change to browse by: physics physics.bio-ph q-bio q-bio.QM References & Citations SciX Google Scholar Semantic Scholar export BibTeX citation Bookmark Bibliographic Tools Bibliographic and Citation Tools Bibliographic Explorer Toggle Bibliographic Explorer ( What is the Explorer? ) Connected Papers Toggle Connected Papers ( What is Connected Papers? ) Litmaps Toggle Litmaps ( What is Litmaps? ) scite.ai Toggle scite Smart Citations ( What are Smart Citations? ) Code, Data, Media Code, Data and Media Associated with this Article alphaXiv Toggle alphaXiv ( What is alphaXiv? ) Links to Code Toggle CatalyzeX Code Finder for Papers ( What is CatalyzeX? ) DagsHub Toggle DagsHub ( What is DagsHub? ) GotitPub Toggle Gotit.pub ( What is GotitPub? ) Huggingface Toggle Hugging Face ( What is Huggingface? ) ScienceCast Toggle ScienceCast ( What is ScienceCast? ) Demos Demos Replicate Toggle Replicate ( What is Replicate? ) Spaces Toggle Hugging Face Spaces ( What is Spaces? ) Spaces Toggle TXYZ.AI ( What is TXYZ.AI? ) Related Papers Recommenders and Search Tools Link to Influence Flower Influence Flower ( What are Influence Flowers? ) Core recommender toggle CORE Recommender ( What is CORE? ) Author Venue Institution Topic About arXivLabs arXivLabs: experimental projects with community collaborators arXivLabs is a framework that allows collaborators to develop and share new arXiv features directly on our website. 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