Influence of Hydration Status on the Yield of Injectable Platelet-Rich Fibrin

Due to the current lack of evidence regarding the influence of fluid intake on the amount of leukocyte- and platelet-rich fibrin fraction present in blood samples, this study aims to investigate the positive influence of this factor on the body's own matrix. Platelet-rich fibrin, a fibrin-rich autologous matrix obtained from autologous blood via centrifugation, contains not only fibrin but also important growth factors and a large number of white blood cells, all of which make a significant contribution to tissue regeneration. The obtained fibrin matrix can be used as a membrane, as a clot (A-PRF), or as a liquid (i-PRF) to positively influence the healing process. The novelty of this work lies in shifting the focus from purely technical centrifugation parameters to a patient-centered, easily modifiable biological variable. Hydration status represents a low-cost, non-invasive, and immediately applicable strategy to optimize autologous blood concentrates. These findings are directly relevant to clinicians seeking to enhance the predictability and efficiency of PRF-based regenerative procedures in implant dentistry.