Effect of Supplementation With Anthocyanins on Iron Metabolism in Female Swimmers

The study included 8-weeks supplementation of black chokeberry extract with 18% standardization of the anthocyanins content (dose 2×200 mg per day), or a placebo product that was made from chokeberry fiber. Purpose: The aim of the study is effectiveness check of black chokeberry intake as a potential factor which, due to its ability to reduce inflammation and chelate iron ions, will have an impact on minimizing undesirable changes in the red blood cell system. Hypothesis: Main hypothesis: Supplementation of black chokeberry (Aronia melanocarpa) has a positive effect on iron economy of female swimmers. Auxiliary hypotheses: 1. Supplementation of black chokeberry enhances the antioxidant status and has a positive effect on the prooxidant-antioxidant balance. 2. Supplementation of black chokeberry reduce inflammation induced by intensive exercises, by reducing inflammatory markers. Study plan: Before first term of the study: Control of Menstrual Cycle Phases since March 2023 (installing the application on the phone - Menstrual Period Tracker calendar). First term of the study: 1. Menstrual cycle control survey. 2. Conduct a body composition analysis. 3. Collection of blood samples before the exercise test for biochemical determinations. 4. Exercise test in the swimming pool - crawl style intensive test (800 m + 200 m + 50 m). 5. Collection of blood immediately after exercise and 3 hours later. 6. Supplementation for nearly 56 days, depending on the length of the menstrual cycle. Second term of the study: Repeat the measurements from the 1st date of test. Participants: The group of selective and homogeneous female swimmers, who train swimming intensively and attend the Sports Championship School Complex.