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Engineering a Compact Prime Editor for Single-AAV Delivery

Papavassiliou, Alex et al.
ScholarlyCommons · Papers
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chemical-engineeringcrisprgene-editing
Prime editing enables precise genomic modifications without generating double-strand breaks, but conventional SpCas9-based prime editors exceed the ~4.7 kb packaging capacity of a single adeno-associated virus (AAV) vector. Dual-AAV strategies circumvent this limitation, but suffer from reduced edit
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