Benchmarking immunoinformatic repertoire assemblies from bulk RNA-seq and PCR-based V(D)J sequencing using PCR-free SMRT RNA sequencing

Immune repertoire sequencing (RepSeq) is a crucial immunomonitoring tool, requiring high sensitivity, accuracy, and minimal bias. Although V(D)J mRNA sequencing using 5′ rapid amplification of cDNA ends (RACE-RepSeq) is broadly used, its reliance on PCR may create distortions. Alternatively, in sili