Transcriptome analysis of 3×Tg-AD mice exacerbated by HFD reveals novel molecular targets of Dendrobine protection | Zenodo Skip to main Communities My dashboard Log in Sign up Published April 25, 2026 | Version v1 Dataset Open Transcriptome analysis of 3×Tg-AD mice exacerbated by HFD reveals novel molecular targets of Dendrobine protection Authors/Creators Liu, Bo (Researcher) 1 Mao, Qianhua (Researcher) 1 Liu, Jie (Researcher) 1 Show affiliations 1. Zunyi Medical University Contributors Data collector: Mao, Qianhua 1 Data curator: Liu, Jie 1 Project leader: Liu, Bo 1 Show affiliations 1. Zunyi Medical University Description Abstract: Background: The prevalence of Alzheimer's disease (AD) is increasing globally, and the progression of (AD) is now known to be exacerbated by high-fat diet (HFD). Dendrobine, an active alkaloid of Dendrobium nobile Lindl . , is effective against AD models. This study aimed to examine the effects of Dendrobine on AD-HFD comorbidity and used RNA-Seq to explore the molecular targets. Methods: The AD-HFD comorbidity model was established by feeding 4-month-old 3 × Tg-AD mice with HFD for 5 months. Dendrobine (1, 10 and 20 mg/kg, po) was administered staring 6-month-old for 3 months, and age-matched 3 × Tg-AD mice fed normal diet were used as Controls. Behavioral tests were performed in 9-month-old mice, followed by histopathology. Hippocampal RNA was isolated and subjected to RNA-Seq and RT-qPCR analyses. Results: Model mice exhibited more serious cognition deficits evidenced by Y maze , Novel object recognition, Open field, and Elevated plus maze. Nissl bodies in the hippocampus and cortex were decreased, while t he expression of p-Tau (Thr231) increased . Dendrobine treatments ameliorated behavioral deficits and improved morphological lesions. RNA-Seq revealed distinct gene expression patterns among groups. Differentially expressed genes in Model mice were attenuated by Dendrobine and RT-qPCR verified selected genes . Ingenuity pathway analysis revealed metabolism disruption including downregulation of lipid metabolism and upregulation of “Mitochondrial Dysfunction”. AD-HFD increased upstream regulators related to oxidative stress and inflammation, while decreased molecules related to transcription factors for metabolism regulation. Dendrobine attenuated or reversed these molecular changes. Conclusions: AD-HFD comorbidity produced severer pathology than AD alone. Dendrobine is effective against behavioral, pathological, and transcriptomic changes in Model mice, probably through targeting aberrant lipid metabolism, inflammation, and neurodegeneration in AD-HFD comorbidity mice. Files Files (6.2 MB) Name Size Download all AD+HFD-5 groups in 15 samples.xlsx md5:5bbc6daeacf97aa52ddcd55cc78afa3e 6.2 MB Download Additional details Funding National Natural Science Foundation of China 82560832 Dates Submitted 2026-04-25 in reversion Software Development Status Active 18 Views 11 Downloads Show more details All versions This version Views Total views 18 18 Downloads Total downloads 11 11 Data volume Total data volume 68.6 MB 68.6 MB More info on how stats are collected.... Versions External resources Indexed in OpenAIRE Communities Keywords and subjects Keywords 3×Tg-AD mice; high-fat diet; AD-HFD comorbidity; Dendrobine; RNA-Seq; Ingenuity pathway analysis Details DOI DOI Badge DOI 10.5281/zenodo.19759886 Markdown [](https://doi.org/10.5281/zenodo.19759886) reStructuredText .. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.19759886.svg :target: https://doi.org/10.5281/zenodo.19759886 HTML <a href="https://doi.org/10.5281/zenodo.19759886"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.19759886.svg" alt="DOI"></a> Image URL https://zenodo.org/badge/DOI/10.5281/zenodo.19759886.svg Target URL https://doi.org/10.5281/zenodo.19759886 Resource type Dataset Publisher Zenodo Published in Scientific Report, ISSN: 2045-2322, 2026. Languages English Rights License Creative Commons Attribution 4.0 International The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited. Read more Citation Export Technical metadata Created April 25, 2026 Modified April 25, 2026 Jump up About About Policies Infrastructure Principles Projects Roadmap Contact Blog Blog Support Help FAQ Developers REST API OAI-PMH Contribute GitHub Donate Funded by Powered by CERN Data Centre & InvenioRDM Status Privacy policy Cookie policy Terms of Use This site uses cookies. Find out more on how we use cookies Accept all cookies Accept only essential cookies