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A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes

Sidik, Saima M et al.
eScholarship · Papers
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31-biological-sciences-for-20203202-clinical-sciences-for-2020
Apicomplexan parasites are leading causes of human and livestock diseases such as malaria and toxoplasmosis, yet most of their genes remain uncharacterized. Here, we present the first genome-wide genetic screen of an apicomplexan. We adapted CRISPR/Cas9 to assess the contribution of each gene from t
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