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Computational and experimental engineering of a Pleurotus citrinopileatus lipases : structural insights and functional optimization to adapt the hydrolytic profile for cheese applications

Henrich, Lea et al.
JLUpub · Papers · License: CC BY 4.0
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Mutants of a golden oyster mushroom lipase (Pleurotus citrinopileatus; PCI_Lip), were engineered to enhance hydrolysis profiles for cheese production. Key residues affecting activity were identified by SMME (Structure model based on AlphaFold3 prediction, followed by Molecular docking, Molecular dyn
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