Testing Reciprocal Formation of Sequence-Bearing Molecules within Chemical Organization: An Integrated Experimental Protocol for the Synechite Framework

The Synechite framework proposes that sequence-based heredity should be investigated as a possible outcome of chemically active organization and its continuing interactions—not as a genetic system that must first appear independently and only later become integrated into a chemical community. The proposed mechanism is reciprocal: a localized, dynamically maintained organization changes which molecular components form, persist or interact; some resulting components alter the organization and the chemical conditions of their own subsequent production or retention; feedback can then change the distribution of later products. If this reciprocal process eventually produces sequence-dependent copying and transferable variation, sequence-based heredity may become an organizational capability of the system. This is a hypothesis to test, not an established account of the historical origin of life. The preceding protocol used a chemically fuelled dynamic combinatorial library (DCL) as the principal starting platform. That system is useful for studying selection and feedback, but its oligomers are synthetic analogues rather than RNA, and the published template-selection experiments supply a nucleic-acid template. It is therefore not the most direct first test of how sequence-bearing genetic polymers can form in the first place. A more relevant experimental baseline has since become available: a 2026 study reports short oligomers from non-activated nucleotide building blocks under hot, acidic wet–dry cycling without added catalysts. The reported products were mostly dimers, extended to tetramers, and reached yields up to approximately 3%; the study also emphasizes the risk of mistaking non-covalent nucleotide clusters for covalent oligomers. It does not demonstrate mixedsequence heredity, and that limitation defines an important experimental opportunity. This proposal therefore centers on one integrated experiment: expose a chemically characterized mixture of nucleotide building blocks to a controlled non-equilibrium organization—initially wet–dry cycling with a validated organizing interface or matrix—and determine whether (i) covalent, sequence-bearing oligomers form without supplying their sequences as templates; (ii) the organization causally changes their formation, distribution or persistence; and (iii) the resulting oligomer population in turn changes the organization or the next reaction cycle. The primary system should be compared against matched unorganized and inactive controls. Oligomers must be chemically verified and sequence order resolved to the level claimed. The same experimental program includes reciprocal add-back and repeated-cycle tests, rather than treating them as proof of an assumed linear series of natural stages. The goal of the first successful result is not to claim that life has been created or that heredity is already established. It is to demonstrate, or falsify in the selected system, a coupled mechanism for organizationally influenced formation and feedback of sequence-bearing molecular products. Demonstrating sequence-based heredity requires the additional evidence that a sequence-dependent difference can be copied or otherwise specifically transmitted across cycles with measurable fidelity. The protocol separates those evidentiary standards without presuming that the underlying natural process was temporally divided into separate stages. It includes controls, analytical requirements, stop rules and an invitation for collaboration among prebiotic chemists, systems chemists, surface/interface researchers, analytical chemists and origin-of-life laboratories.